Holders Liverpool crashed out of the Champions League in the last 16 after Atletico Madrid scored three goals in extra time to earn a shock 3-2 victory at Anfield on Wednesday that sent them through 4-2 on aggregate.
After falling 2-0 down on the night, substitute Marcos Llorente struck twice in extra time and Alvaro Morata added a late third to ensure a famous victory for Diego Simeone's side against a Liverpool team unbeaten in their last 25 home games in European competition.
Premier League leaders Liverpool enjoyed the greater possession, created more chances, drew a series of saves from Atletico keeper Jan Oblak and scored through Georginio Wijnaldum and Roberto Firmino.
Yet after last season's memorable comeback by Liverpool against Barcelona in the semifinals, it was the Spaniards who turned the tables this time.
Simeone's Atletico are famed for their defensive solidity and their superb organisation and those qualities were on full display, but it was their clinical finishing and remarkable spirit that proved the key elements.
"We don't know where our limit is," said Llorente who had only scored once for Atletico before this game.
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"We all defended until the death and when a team all digs in like this, things like this happen. I cannot describe this feeling."
Liverpool manager Jurgen Klopp was not impressed with Atletico's approach, however.
"I don't understand with the quality they have the football they play. They could play proper football but they stand deep and have counter-attacks," said the German.
"We accept it of course but it doesn't feel right tonight. I realise I am a really bad loser, especially when the boys put such an effort in against world class players on the other side who defend with two rows of four."
Atleti boss Simeone was curt in his response to Klopp's reaction.
"[We play] to win. With our weapons, to win," he said. "Respecting our identity, respecting the characteristics of the players, exploiting the defects of the opponents, that's how we play."
After allowing an early chance for Diego Costa, which the former Chelsea forward drove into the side-netting, Liverpool, trailing 1-0 from the first leg, laid siege to the Atletico goal.
The home team broke through two minutes before halftime when Alex Oxlade-Chamberlain burst to the byline and pulled back a cross to Wijnaldum who powered an angled header into the bottom corner.
There was little change to the pattern of the game after the interval with Liverpool peppering Oblak's goal but the Slovenian proved himself up to the task, denying efforts from Mohamed Salah, Firmino and Sadio Mane while Andy Robertson headed against the bar.
"Oblak is the best goalkeeper in the world, I have no doubts," Simeone said after the match. "[Lionel] Messi resolves games offensively, and Oblak does it in goal.
"The fans will remember this game for a long time."
It took just four minutes for Liverpool to take the lead in the tie with Firmino heading against the post and driving home the loose ball as Anfield got ready for a night of celebration.
Yet then it all went wrong for Klopp's side.
Goalkeeper Adrian, standing in for the injured Alisson Becker, passed the ball out straight to Joao Felix who fed Llorente and the substitute punished the Spanish keeper with a precise shot into the far corner.
Llorente produced a carbon-copy finish following good work on the counter from Morata to end Liverpool's hopes of defending their European crown.
Morata put the icing on the cake in the final moments with a cool finish after Liverpool had thrown all their players forward.
"We always believed. I was saying to some the lads, they've never played here before, it's very difficult to play here with the team, the fans, and I thought defensively it was outstanding," said Kieran Trippier, who joined Atleti from the Tottenham Hotspur team that lost to Liverpool in last season's Champions League final.
"We kept believing and we had to be clinical because you don't get many chances at Anfield.
"You see how we played tonight and that's how we train every session with the Mister [Simeone], you see him on the touchline, that's what he's like in training, he gives you that lift.
"He's relentless on the touchline. It's little things, when you look over it just gives you that lift. It's a joy to work with him every single day and I'm delighted to be playing for him."
The pain of this exit will surely ease for Liverpool, who are 25 points clear in the Premier League and will soon win their first domestic league title for 30 years.
But for Atletico, who have never won Europe's premier club competition, this was a triumph which will have them dreaming of even greater things.
"This will go down in our history," said Simeone. "It fills us with pride as a club, to be once more one of the top eight clubs in Europe."
ESPN football correspondent Alex Kirkland contributed to this report.